STUDIES ON THE UPTAKE AND BINDING OF 5α-DIHYDROTESTOSTERONE BY RAT VENTRAL PROSTATE CELL NUCLEI IN VITRO

in European Journal of Endocrinology
Author: Olav Unhjem
View More View Less
Restricted access

ABSTRACT

Following incubation of rat ventral prostate homogenates with 1,2-3H]-5α-dihydrotestosterone, the steroid was taken up and bound by the nuclei. Sodium chloride extraction of the nuclei removed macromolecules which might at least be partially responsible for the binding. Purified nuclei which were incubated with the radioactive steroid in either Tris-EDTA buffer or a 105 000 × g supernatant fraction prepared from the organ showed a lower degree of binding. Gel filtration chromatography of sodium chloride extracts from these nuclei disclosed binding of steroids to macromolecules. In all the experiments the steroids bound by nuclei consisted of unchanged 5α-dihydrotestosterone.

 

     European Society of Endocrinology

Sept 2018 onwards Past Year Past 30 Days
Abstract Views 99 27 3
Full Text Views 40 0 0
PDF Downloads 22 0 0