Peripheral blood mononuclear cells preferentially activate 11-oxygenated androgens

Objective Androgens are important modulators of immune cell function. The local generation of active androgens from circulating precursors is an important mediator of androgen action in peripheral target cells or tissues. We aimed to characterize the activation of classic and 11-oxygenated androgens in human peripheral blood mononuclear cells (PBMCs). Methods PBMCs were isolated from healthy male donors and incubated ex vivo with precursors and active androgens of the classic and 11-oxygenated androgen pathways. Steroids were quantified by liquid chromatography-tandem mass spectrometry. The expression of genes encoding steroid-metabolizing enzymes was assessed by quantitative PCR. Results PBMCs generated eight-fold higher amounts of the active 11-oxygenated androgen 11-ketotestosterone than the classic androgen testosterone from their respective precursors. We identified the enzyme AKR1C3 as the major reductive 17β-hydroxysteroid dehydrogenase in PBMCs responsible for both conversions and found that within the PBMC compartment natural killer cells are the major site of AKRC13 expression and activity. Steroid 5α-reductase type 1 catalyzed the 5α-reduction of classic but not 11-oxygenated androgens in PBMCs. Lag time prior to the separation of cellular components from whole blood increased serum 11-ketotestosterone concentrations in a time-dependent fashion, with significant increases detected from two hours after blood collection. Conclusions 11-Oxygenated androgens are the preferred substrates for androgen activation by AKR1C3 in PBMCs, primarily conveyed by natural killer cell AKR1C3 activity, yielding 11-ketotestosterone the major active androgen in PBMCs. Androgen metabolism by PBMCs can affect the results of serum 11-ketotestosterone measurements, if samples are not separated in a timely fashion. Significance statement We show that human peripheral blood mononuclear cells (PBMCs) preferentially activate 11-ketotestosterone rather than testosterone when incubated with precursors of both the classic and the adrenal-derived 11-oxygenated androgen biosynthesis pathways. We demonstrate that this activity is catalyzed by the enzyme AKR1C3, which we found to primarily reside in natural killer cells, major contributors to the anti-viral immune defense. This potentially links intracrine 11-oxygenated androgen generation to the previously observed decreased NK cell cytotoxicity and increased infection risk in primary adrenal insufficiency. In addition, we show that PBMCs continue to generate 11-ketotestosterone if the cellular component of whole blood samples is not removed in a timely fashion, which could affect measurements of this active androgen in routine clinical biochemistry.


Introduction
A critical step in androgen activation is the conversion of the androgen precursor androstenedione (A4) to the active androgen testosterone (T) (1,2,3), which occurs in the testes catalyzed by 17β-hydroxysteroid dehydrogenase type 3 (HSD17B3).However, in the majority of peripheral target tissues of androgen action, the intracrine activation of A4 to T is catalyzed by the enzyme AKR1C3 (aldoketo reductase 1C3, also termed 17β-hydroxysteroid dehydrogenase type 5) (1,2,3), which thereby represents a key regulator of local androgen exposure.Peripheral blood mononuclear cells (PBMCs) can activate A4 to T (4, 5) catalyzed by AKR1C3 (6).Androgens impact on a multitude of immune cell functions, including proliferation, differentiation, apoptosis, cytokine and immunoglobulin productions (7,8) and also link immune and metabolic regulation (9).
Of note, a number of studies have shown gradual increases in T measured in serum, if the blood sample is left unseparated from cellular components for an increased period of time, using either immunoassays (19,20,21) or ultra-high performance liquid chromatographytandem mass spectrometry (LC-MS/MS) for steroid quantification (22,23).One of those studies additionally investigated the effect of time before separation on 11KT concentrations measured in serum and observed a much more pronounced increase in 11KT than T over time (23), which could have a differential impact on the measurement of circulating 11-oxygenated androgen concentrations.
In this study, we explore this further and undertake a detailed characterization of androgen activation via both classic and 11-oxygenated androgen biosynthesis pathways in the human PBMC compartment and subpopulations, utilizing PBMCs isolated from healthy volunteers.

Blood collection
The collection of blood for PBMC isolation and serum steroid analysis underwent ethical approval by the Science, Technology, Engineering and Mathematics Ethical Review Committee of the University of Birmingham (ERN_17-0494, ERN_17-0494A, and ERN_14-0446).All participants gave informed, written consent prior to study participation.Exclusion criteria for participation were any acute or chronic disease affecting steroid biosynthesis or metabolism and intake of any medication known to interfere with steroid biosynthesis or metabolism.All blood samples were collected between 09:00 h and 11:00 h; 50 mL of blood were collected into K 2 EDTA Vacutainers™ (purple top) for the immediate isolation of PBMCs.
To assess the effect of the time period between blood collection and separation of the cellular components by centrifugation on the measured concentrations of serum steroids, blood was collected from healthy volunteers (3 male, 3 female; age range: 28-50 years; BMI range 20.8-31.1 kg/m 2 ) into several SST™ Vacutainers™ (gold top).The tubes were left unseparated at room temperature in an air-conditioned room and were separated by centrifugation (2000 g, 10 min) after defined time periods (0, 1, 2, 4, 6 and 24 h).The separated serum was stored at −80°C.

Isolation of peripheral blood mononuclear cells
PBMCs were isolated from whole blood by density gradient centrifugation using Ficoll Paque Plus (GE Healthcare) following the manufacturer's instructions.Isolated PBMCs were washed in RPMI-1640 (Sigma-Aldrich) supplemented with 100 U/mL penicillin and 0.1 mg/mL streptomycin, counted and assessed for viability by trypan blue exclusion.A minimum of 3 × 10 6 cells was frozen in 400 μL TRI reagent ® (Sigma Aldrich) and stored at −80 °C for RNA analysis at a later date.

RNA extraction and quantitative PCR
Samples stored in TRI reagent ® were defrosted and the RNA in the aqueous phase of the phenol-chloroform extraction was purified using the RNeasy Mini Kit (Qiagen).RNA concentrations were determined from the absorbance of the sample at 260 nm using a Nanodrop spectrophotometer and reverse transcription (RT) was performed using Applied Biosystems™ TaqMan™ RT Reagents following the manufacturer's protocol.Quantitative PCR (qPCR) was performed on an ABI 7900HT sequence detection system (Perkin Elmer, Applied Biosystems) using TaqMan™ Gene Expression Assays (FAM-labeled) and the SensiFAST™ Probe Hi-ROX kit (Bioline).∆Ct was calculated as Ct (Target)-Geometric mean (Ct (HPRT1), Ct (GAPDH)).Gene expression in arbitrary units (A.U.) was calculated as 1000*2 -∆Ct .For targets not reproducibly detected in duplicate reactions, relative gene expression is shown as 0.

Ex vivo steroid metabolism assays in peripheral blood mononuclear cells
For steroid metabolism assays, 3 × 10 6 cells were incubated with 100 nM of the respective steroid in a final volume of 500 μL RPMI-1640 supplemented with penicillin and streptomycin.Steroids were added from stock solutions in methanol.The final methanol concentration in the incubations was 0.00304% (v/v).Technical replicates were prepared, if sufficient cell numbers were available.During the incubation, the samples were constantly gently rotated at 37°C for 24 h.For each experiment, incubations of cells with methanol only and cell-free incubations for all steroids were prepared as controls.Additionally, dilutions of each steroid at 100 nM in medium were frozen at −20°C immediately after preparation.At the end of the incubation period samples were centrifuged for 2 min at 9,400 rpm.The supernatant was stored at −20°C for steroid analysis.The cell pellet was washed in PBS, suspended in 50 μL lysis buffer (50 mM Tris pH 8.0, 150 mM NaCl, 0.1% SDS, 0.5% sodium deoxycholate, 1% Trition X-100, 0.1 mM DTE, 0.1 mM PMSF, 0.1 mM EDTA) and stored at −80°C for protein quantification.

Analysis of published RNAseq data from PBMC subpopulations
We accessed dice-database.org(25) to investigate AKR1C3 expression determined by RNAseq in FACS-sorted PBMC subpopulations.The database contains expression data from 54 male and 37 female healthy donors (age range 18-61 years).AKR1C3 expression data were downloaded and plotted in GraphPad Prism 8 as log 2 transformed transcripts per million (TPM).

Natural killer cell ex vivo steroid metabolism assays
PBMCs were prepared as described above and NK cells were subsequently enriched using the MACS ® human NK cell isolation kit (Miltenyi Biotec) as per the manufacturer's instructions.Cells were checked for viability, counted and steroid conversion assays were set up as described above.

Statistical analysis
Changes in serum steroid concentrations were analyzed in GraphPad Prism 8 using ANOVA followed by a Dunnett multiple comparison test to compare each timepoint against the sample processed immediately after collection (0 h). Statistical analysis of differences in steroid concentrations in the supernatants from the PBMC ex vivo incubations was performed by Wilcoxon matched-pairs signed-rank test or Mann-Whitney test as appropriate.Statistical analysis of RNAseq data was performed by oneway ANOVA followed by Tukey's multiple comparisons test or Mann-Whitney test as appropriate.

Androgen activation in PBMCs is primarily catalyzed by AKR1C3 and SRD5A1
Using qPCR, we identified AKR1C3 as the major reductive, activating 17β-hydroxysteroid dehydrogenase isoform in PBMCs, while HSD17B3, generally considered a testesspecific isoform, was expressed at very low levels only (Fig. 1A).While we could not detect the expression of steroid 5α-reductase type 2 (SRD5A2) and 17β-hydroxysteroid dehydrogenase type 2 (HSD17B2) in any of the samples from 14 donors using our qPCR assay, we detected consistently high expression of steroid 5α-reductase type 1 (SRD5A1) in samples from all donors (n = 14) and detected 17β-hydroxysteroid dehydrogenase type 4 (HSD17B4) expression in samples from 6 of 14 donors.This indicates that SRD5A1 is responsible for the 5α-reduction we observe in our PBMC incubations with androgen substrates while HSD17B4 catalyzes the oxidative, inactivating 17β-hydroxysteroid dehydrogenase activity we observed (Fig. 1A), consistent with previously published findings (6).11β-hydroxysteroid dehydrogenase type 1 (HSD11B1) expression was detectable in samples from 13/14 donors, while HSD11B2 mRNA was not detected.Figure 1B schematically illustrates the enzymes identified by qPCR as present in PBMCs and their roles in androgen activation and inactivation in the classic and 11-oxygenated androgen biosynthesis pathways.

PBMCs preferentially activate 11-oxygenated androgens
In order to unravel the pathways of androgen metabolism in PBMCs, we isolated PBMCs from male healthy donors and performed ex vivo incubations, using as substrates several androgen precursors and active androgens from both the classic (DHEA, A4 and T) and 11-oxygenated androgen pathways (11OHA4, 11OHT, 11KA4, 11KT).Product formation was quantified by LC-MS/MS.Initial time-course experiments with PBMCs isolated from three male donors (aged 22, 23 and 28 years) identified 24 h as a suitable incubation time allowing for robust product quantification within the linear range of product formation over time (data not shown).These initial experiments additionally revealed very low 3β-hydroxysteroid dehydrogenase activity in PBMCs and thus excluded DHEA as a relevant substrate for metabolism in PBMCs (data not shown).
All further experiments were performed with PBMCs isolated from men aged 18-30 years (n = 4-5; age 22-30 years; BMI 20.2-29.1 kg/m 2 ) and men aged >50 years (n = 4-7; age 53-72 years; BMI 21.2-30.4kg/m 2 ).We did not observe any significant differences in product formation by PBMCs between the two different age groups (Fig. 2), except a slightly, but not significantly increased median formation of T from A4 and 11KT from 11KA4.Hence, we present and discuss the combined data for the entire cohort below (n = 8-12 per experiment; age range 22-72; BMI range 20.2-30.4kg/m 2 ).
Incubations with A4 and T yielded their respective 5α-reduced products, 5α-dione and DHT, and revealed efficient interconversion of A4 and T by reductive and oxidative 17β-hydroxysteroid dehydrogenases (Fig. 3A  and B).5α-dione and DHT were further converted to their 3α-hydroxy metabolites An and Adiol.
The quantitatively dominant formation observed among all substrates tested was the generation of 11KT from 11KA4, which was significantly higher than the generation of T from A4 (P = 0.001; n = 11; Fig. 3C).After an incubation period of 24 h, PBMCs generated approximately eight times more 11KT than T from their respective precursors 11KA4 and A4 (Fig. 3A and C).However, while T was converted back to A4 in large quantities, incubation with 11KT led to only minor generation of 11KA4 (quantifiable in 8/12 incubations, Fig. 3D), further contributing to the preferential activation of 11KT by PBMCs.
Expressing the observed steady-state between activation and inactivation as product/substrate ratios made this difference even more obvious, clearly indicating that the generation of active 11-oxygenated androgens is favored in PBMCs (Fig. 4A).Product/substrate-ratios for different substrates of 5α-reductase reflect the established substrate preference for SRD5A1 with A4 resulting in the highest activity followed by T and with only minor activity for 11OHA4 (Fig. 4B).
Incubation with the 11-oxygenated androgen precursors 11OHA4 and 11OHT led to only negligible product formation compared to the other substrates tested.After 24 h the generation of the 5α-reduced product 11OH-5α-dione from 11OHA4 could be quantified, as well as the generation of 11KT from 11OHT (Fig. 3E and F).

AKR1C3 expression and activity in PBMCs is primarily located in natural killer cells
In order to identify the subpopulation(s) within the PBMC compartment responsible for the AKR1C3-catalyzed androgen activation observed in our PBMC incubations, we used publicly available RNAseq-based gene expression data from 15 FACS-sorted PBMC subpopulations including B-cells, different T-cell populations, monocytes and natural killer (NK) cells (dice-database.org(25)).This revealed significantly higher AKR1C3 expression in NK cells (P < 0.0001) than in any other PBMC subpopulation (Fig. 5A).There was no difference between AKR1C3 expression in NK cells from male and female donors (Fig. 5B).To confirm NK cells as the major site of AKR1C3 activity, we isolated PBMCs from a leukocyte cone of an anonymous donor and subsequently enriched NK cells in a fraction of the PBMCs.Ex vivo incubations of the matched crude PBMC isolations and the enriched NK cell fraction with the AKR1C3 substrates A4 and 11KA4 confirmed higher AKR1C3 activity in the NK cell-enriched incubation compared to the incubation with crude PBMC isolates (Fig. 5C).

Lag time prior to the separation of cellular components increases 11-ketotestosterone
We collected blood samples from six additional healthy volunteers (3 male, 3 female; age range 28-50 years; BMI range 20.8-31.1 kg/m 2 ) to assess the effect of an extended incubation of their blood samples unseparated from cellular components on the quantification of serum steroids by LC-MS/MS (Fig. 6).We observed a time-dependent increase in the serum concentrations of the AKR1C3 product 11KT, reaching a median relative increase of 44% after 24 h, with significant increases in 11KT already observed after 2 h of leaving the full blood samples unseparated (Fig. 6I).Additionally, we observed decreases in the concentrations of the AKR1C3 substrates A4 and 11KA4, with median relative decreases of 19 and 34%, respectively, at 24 h.The observed decreases in serum A4 concentrations were statistically significant for the majority of time points assessed (P < 0.05), while statistical significance was not reached for the decrease in 11KA4 (Fig. 6C and H).Among the other analytes investigated (DHEA, T, DHT, An, 11OHA4), only 11OHA4 exhibited significant changes over time (P < 0.05), with a median relative decrease of 38% occurring within one hour after blood collection (Fig. 6G).

Discussion
Androgen signaling is vital for immune cell function by regulating proliferation, differentiation, cytokine production and other pathways (7,8).Intracrine androgen activation from adrenal precursors contributes a large proportion of androgen receptor activation in peripheral target cells and tissues (17,26).While we have previously shown that PBMCs can activate the classic androgen precursor A4 to T via AKR1C3 and further convert T to the most potent human androgen DHT (6), we show for the first time in this study that PBMCs preferentially activate the 11-oxygenated androgen precursor 11KA4 to its active counterpart 11KT.We show that PBMCs generate approximately 8-fold more 11KT than T from their respective precursors, revealing 11KT as the predominant active androgen within the human PBMC compartment.Using in vitro systems overexpressing AKR1C3, Barnard et al. (10) previously demonstrated that AKR1C3 has a significantly higher catalytic efficiency for the activation of 11-oxygenated androgens compared to classic androgens, which we have shown here, for the first time, ex vivo in human cells.
In addition, we show that the inactivating conversion of the active androgens T and 11KT to their respective precursors A4 and 11KA4 by oxidative 17β-hydroxysteroid dehydrogenase activity is relevant only for T, while the inactivation of 11KT to 11KA4 occurs only in negligible amounts.Taken together, this demonstrates that PBMCs preferentially activate 11-oxygenated androgens.This observation is in agreement with the study by Barnard et al. (10), who showed that, while HSD17B2 catalyzes the inactivation of T and 11KT with similar efficiencies, increased ratios of AKR1C3 to HSD17B2 favor the activation of 11-oxygenated androgens due to the catalytic preference of AKR1C3 for 11KA4 over A4.
Our results confirm previous findings that steroid 5α-reduction contributes to the activation of classic androgens in PBMCs by generating the most potent human androgen DHT from T (6).The observed substrate preference of 5α-reduction for A4 over T is consistent with the established substrate preference of SRD5A1 (27), which we confirm is the major steroid 5α-reductase in  PBMCs (6).We did not observe relevant 5α-reduction of 11-oxygenated androgens.Using in vitro promoter reporter assays 11KDHT has been shown to activate the androgen receptor with potency and efficacy comparable to DHT.However, it is not clear if the generation of 11KDHT by the 5α-reduction of 11KT is relevant under physiological conditions.Recently, we showed that 11KT metabolism primarily proceeds via the AKR1D1 mediated 5β-reduction of the steroid A-ring and that SRD5A2, but not SRD5A1, can efficiently catalyze the 5α-reduction of 11KT (28).We now confirm that 11KT is not 5α-reduced by human cells with SRD5A1 levels that efficiently convert T to DHT, confirming that the 5α-reduction of 11KT would require the expression of SRD5A2.
In this study, we did not observe a significant effect of age on androgen activation in PBMCs, with only a trend for increased median AKR1C3 activity, while a previous study by our group described significantly increased AKR1C3 and SRD5A1 activities in men aged over 50 years compared to men aged 18-30 years (6).The limited sample numbers and differences in the assays used for steroid quantification (LC-MS/MS in the current study vs thin layer chromatography in the previous one) are likely to be responsible for this discrepancy.However, when assessing the effect of age on androgen activation in peripheral tissues, age-related changes in the supply of androgen precursors from circulation need to be considered, in addition to potential age-dependent changes in the expression of androgen-activating and -inactivating enzymes in the peripheral tissues.While circulating levels of classic androgens significantly decline with age, levels of 11-oxygenated androgens remain constant across adulthood (29,30).Hence, the peripheral activation of 11-oxygenated androgens is favored over the activation of classic androgens not only by the substrate preference of the key androgen-activating enzyme AKR1C3, but also by constant high substrate availability across the life span.Primary adrenal insufficiency is associated with an increased risk of infection compared to the general population (31).In addition, patients with primary adrenal insufficiency show significantly reduced NK cell cytotoxicity (32).Interestingly, this finding is not affected by DHEA replacement therapy excluding the deficiency of the classic androgen pathway precursor DHEA as a cause of the reduced NK cell cytotoxicity.Our study identifies adrenal 11-oxygenated androgen precursors rather than classic androgen precursors as predominant androgens activated in the PBMC compartment and particularly in NK cells, potentially linking the lack of adrenal 11-oxygenated androgen precursors in primary adrenal insufficiency to the observed decrease in NK cell cytotoxicity.
The preference of PBMCs to generate 11KT from 11KA4 via AKR1C3 activity is further reflected in the significant increases in 11KT serum concentrations, if cellular components were not removed from the whole blood samples in a timely fashion, confirming previous preliminary observations (23).We found that these changes became significant after two hours at room temperature without separation, suggesting that blood samples for the measurement of 11-oxygenated androgens should be processed within two hours of collection.11KT is the dominant circulating active androgen in women with polycystic ovary syndrome (15) and congenital adrenal hyperplasia (14,33) and a useful marker for the diagnosis of androgen excess.The ongoing activation of 11KT in unseparated full blood samples suggests that saliva, a cell-free biofluid, could be a superior matrix for the measurement of 11KT (33,34).
In conclusion, we show that 11-oxygenated androgen precursors are the preferred substrates for androgen activation in PBMCs, yielding 11KT as the major active androgen in the PBMC compartment.This is catalyzed by AKR1C3, which is predominantly expressed in NK cells, potentially linking adrenal 11-oxygenated androgen deficiency to the reduced NK cell cytotoxicity in primary adrenal insufficiency.Androgen metabolism by PBMCs can affect the measurement results of 11KT serum concentrations, if the cellular components of whole blood samples are not removed in a timely fashion.

Declaration of interest
Wiebke Arlt is on the editorial board of EJE.Wiebke Arlt was not involved in the review or editorial process for this paper, on which she is listed as an author.

Figure 1
Figure 1 Expression of genes encoding key androgen-metabolizing enzymes in PBMCs isolated from healthy men (A, n = 14; age range 22−72 years; BMI range 20.2-30.4kg/ m 2 ).Gene expression was assessed by quantitative PCR and normalized to HPRT1 and GAPDH expression.Arbitrary units (A.U.) were calculated as 1000 × 2 −∆Ct .(B) Schematic representation of the steroidmetabolizing enzymes in PBMCs and their roles in activation and inactivation of androgens in the classic and 11-oxygenated androgen pathways.

Figure 2
Figure 2 Ex vivo metabolism of classic (A and B) and 11-oxygenated androgens (C, D, E and F) by PBMCs isolated from healthy men aged 18-30 years (n = 4-5; age 22-30 years; BMI 20.2-29.1 kg/m 2 ; solid circles) and men aged >50 years (n = 4-7; age 53-72 years; BMI 21.2-30.4kg/m 2 ; open circles).PBMCs were incubated with 100 nM substrate for 24 h.The respective substrate is shown in a grey box for each graph.Product formation was quantified by LC-MS/MS and normalized to the total protein content of the incubation.Median and range are indicated.Product concentrations below the limit of quantification are shown as 0. Statistical analysis was performed using Mann-Whitney test.

Figure 3
Figure 3 Ex vivo metabolism of classic (A and B) and 11-oxygenated androgens (C, D, E and F) by PBMCs isolated from healthy men (n = 8-12; age range 22-72 years; BMI range 20.2-30.4kg/m 2 ).PBMCs were incubated with 100 nM substrate for 24 h.The respective substrate is shown in a grey box for each graph.Product formation was quantified by LC-MS/MS and normalized to the total protein content of the incubation.Median and range are indicated.Product concentrations below the limit of quantification are shown as 0.

Figure 4
Figure 4 Product/substrate ratios in ex vivo incubations of PBMCs isolated from healthy men (n = 8-12; age range 22-72 years; BMI range 20.2-30.4kg/m 2 ) after the addition of different steroid substrates.(A) Steady-state ratios of 17β-hydroxysteroid dehydrogenase products and substrates, reflecting the dominant contribution of the reductive, activating 17β-hydroxysteroid dehydrogenase AKR1C3 to 11-oxygenated androgen metabolism.(B) 5α-reductase activity by SRD5A1.Product and substrate were quantified by LC-MS/ MS after a 24-h incubation.Ratios were normalized to the total protein content of the incubation.Statistical analysis was performed using the Wilcoxon matched-pairs signed-rank test.

Figure 5
Figure 5 Natural killer cells are the major peripheral blood mononuclear cell population with AKR1C3 expression (A and B) and activity (C).RNAseq analysis of FACS-sorted PBMC subpopulations (dice-database.org;(25)) revealed natural killer cells (NK cells) as the cell type with the highest AKR1C3 expression (A) with comparable expression in women (n = 37) and men (n = 54) (B).NK cell-enriched incubations with AKR1C3 substrates (n = 4 technical replicates) showed higher AKR1C3 activity (shown as product/substrate ratio) compared to incubations of crude PBMC preparations from the same donor (n = 1) (C).NK cells enriched using MACS ® negative selection (Miltenyi Biotec) were incubated with 100 nM substrate and product formation after 24 h was analyzed by LC-MS/MS.Statistical analysis of AKR1C3 expression in the PBMC subpopulation was performed by one-way ANOVA followed by Tukey's multiple comparisons test.AKR1C3 expression in male and female NK cells was compared by Mann-Whitney test.TPM, transcripts per million.

Figure 6
Figure 6 Relative changes in androgen concentrations compared to baseline, measured in serum after incubation of unseparated whole blood samples.Baseline samples were separated by centrifugation within one hour after collection.The remaining samples were incubated as whole blood at room temperature for the times indicated prior to the separation of serum and cellular content (sample numbers: n = 6 for DHEA (B), n = 6 for A4 (C), n = 5 for T (D), n = 3 for DHT (E), n = 6 for An (F), n = 6 for 11OHA4 (G), 11KA4 (H) and 11KT (I)).Individual data points are shown with range and median.Statistical analysis was performed on the concentrations in nM using ANOVA, followed by Dunnett multiple comparison test to compare each time point against baseline.
This work was supported by the Wellcome Trust (Investigator Grant WT209492/Z/17/Z, to W A), the Academy of Medical Sciences UK (Newton Advanced Fellowship NAF004\1002, to K H S) and Diabetes UK (RD Lawrence Fellowship, to I A).WA receives support from the NIHR Birmingham Biomedical Research Centre at the University Hospitals Birmingham NHS Foundation Trust and the University of Birmingham (Grant Reference Number BRC-1215-20009).The views expressed are those of the authors and not necessarily those of the NIHR UK or the Department of Health and Social Care UK.