MORPHOLOGICAL STUDIES OF TRANSCRIPTION

in European Journal of Endocrinology
Restricted access

ABSTRACT

Preparative techniques are detailed for isolating nuclear contents of amphibian oocytes and HeLa cells for electron microscopy, and the ultrastructure of presumptive ribosomal RNA (rRNA) genes and other active chromosomal loci is described. The rRNA genes from the two cell types present strikingly similar structural configurations, both being fully loaded with RNA polymerases and exhibiting gradients of short to long nascent ribonucleoprotein (RNP) fibrils. Other chromosomal loci in the two cell types are strikingly different; those on lampbrush chromosomes of amphibian oocytes show closely-spaced polymerases and extremely long RNP fibrils, whereas those on HeLa genomes have widely-spaced polymerases and relatively short attached RNP fibrils.

 

     European Society of Endocrinology

Related Articles

Article Information

Metrics

All Time Past Year Past 30 Days
Abstract Views 498 498 15
Full Text Views 55 51 1
PDF Downloads 20 19 2

Altmetrics

Cited By

PubMed

Google Scholar