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Sodium/proton exchange in FRTL-5 thyroid cells: Role in the maintenance of intracellular pH and TSH-stimulated cell growth
in European Journal of EndocrinologyIn eukaryotes cytoplasmic pH is an important determinant of many intracellular processes and has been implicated as a regulatory signal in several cellular events (Nuccitelli & Deamer 1981). The regulation of intracellular pH (pHi) largely depends on an electroneutral, amiloride-sensitive, Na+/H+ exchange in the plasma membrane (Nuccitelli & Deamer 1981). It has recently been suggested that a stimulation of Na+/H+ exchange, producing a rapid and persistent alkalinization is associated with mitogen action in vertebrate cells (Moolenar 1986).
FRTL-5 is a continuous strain of functioning rat thyroid cells, which require TSH for their growth (Ambesi-Impiombato et al. 1980). In the present study we describe an amiloride-sensitive Na+/H+ exchange system in these cells, and we show that it plays an important role in the regulation of pHi. The pHi has been measured using the equilibrium distribution of 14C-labelled 5,5-[2-14C]dimethyl - oxazolidine -2,4 - dione (DMO) (Waddel & Butler
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